recipient strain s pyogenes atcc 12202 (ATCC)
Structured Review

Recipient Strain S Pyogenes Atcc 12202, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 48 article reviews
Images
1) Product Images from "Conjugative Transfer and cis -Mobilization of a Genomic Island by an Integrative and Conjugative Element of Streptococcus agalactiae"
Article Title: Conjugative Transfer and cis -Mobilization of a Genomic Island by an Integrative and Conjugative Element of Streptococcus agalactiae
Journal: Journal of Bacteriology
doi: 10.1128/JB.02199-12
Figure Legend Snippet: Strains and plasmids used in this study
Techniques Used: Plasmid Preparation, Mutagenesis
Figure Legend Snippet: Analysis of eight clones of transconjugants obtained after filter-mating experiments between donor strain 515 (carrying ICE_515_tRNALys) and recipient strain S. pyogenes ATCC 12202. (A) Amplification of the right junction of the ICEs in these clones as for donor cells, using primers specific of the integrase genes and of the tRNALys gene (lane D). The negative control is S. pyogenes ATCC 12202, used as recipient cells (lane R). Lane M shows DNA molecular weight marker. (B) Analysis of the transconjugants by pulsed-field gel electrophoresis in parallel to the donor strain (lane D) and the recipient strain (lane R).
Techniques Used: Clone Assay, Amplification, Negative Control, Molecular Weight, Marker, Pulsed-Field Gel, Electrophoresis